Review



celltrace calcein red-orange, am  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Thermo Fisher celltrace calcein red-orange, am
    Celltrace Calcein Red Orange, Am, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/celltrace+calcein+red-orange/celltrace+calcein+red+orange+am/pm40020762-299-0-14
    Average 90 stars, based on 1 article reviews
    celltrace calcein red-orange, am - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
    Article Snippet: On the day of photoactivation, cells were stained with SPACECAT molecules (e.g., calcein NVOC for Fig. ; calcein NVOC, PA-JF549, PA-JF646 for Fig. ) as described in “SPACECAT staining.” For characterization of SPACECAT’s spatial resolution and temporal stability (Figs. and ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Cas9-mediated knockout of Ndrg2 enhances the regenerative potential of dendritic cells for wound healing.
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: Chemical compound, drug , CellTrace Calcein Red-Orange , ThermoFischer , C34851 , (125 nM).

    Article Title: Alpha-synuclein fragments trigger distinct aggregation pathways
    Article Snippet: CellTrace Calcein red-orange (Thermo Fisher Scientific) was used as a cell filling dye prior to fixing with 4% paraformaldehyde (Sigma-Aldrich).

    Article Title: Spatially-Resolved Live Cell Tagging and Isolation Using Protected Photoactivatable Cell Dyes
    Article Snippet: For characterization of SPACECAT’s spatial resolution and temporal stability ( , ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Regulation of sedimentation rate shapes the evolution of multicellularity in a close unicellular relative of animals
    Article Snippet: For cytoplasmic staining of cells in , cells were either stained with CellTrace CFSE Cell Proliferation Kit (Thermofisher) or CellTrace Calcein Red-Orange (Thermofisher).

    Article Title: Antagonistic Inflammatory Phenotypes Dictate Tumor Fate and Response to Immune Checkpoint Blockade
    Article Snippet: Following adhesion for 2h, cells were stained in the wells with 0.5 μM CellTrace Calcein Green (ThermoFisher Scientific) for 20 min. NK cells were stained separately with 0.32 μM CellTrace Calcein Red-Orange (ThermoFisher Scientific) for 20 min.

    Article Title: Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    RNA Sequencing Assay:

    Article Title: Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
    Article Snippet: On the day of photoactivation, cells were stained with SPACECAT molecules (e.g., calcein NVOC for Fig. ; calcein NVOC, PA-JF549, PA-JF646 for Fig. ) as described in “SPACECAT staining.” For characterization of SPACECAT’s spatial resolution and temporal stability (Figs. and ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Cas9-mediated knockout of Ndrg2 enhances the regenerative potential of dendritic cells for wound healing.
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: Chemical compound, drug , CellTrace Calcein Red-Orange , ThermoFischer , C34851 , (125 nM).

    Article Title: Alpha-synuclein fragments trigger distinct aggregation pathways
    Article Snippet: CellTrace Calcein red-orange (Thermo Fisher Scientific) was used as a cell filling dye prior to fixing with 4% paraformaldehyde (Sigma-Aldrich).

    Article Title: Spatially-Resolved Live Cell Tagging and Isolation Using Protected Photoactivatable Cell Dyes
    Article Snippet: For characterization of SPACECAT’s spatial resolution and temporal stability ( , ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Regulation of sedimentation rate shapes the evolution of multicellularity in a close unicellular relative of animals
    Article Snippet: For cytoplasmic staining of cells in , cells were either stained with CellTrace CFSE Cell Proliferation Kit (Thermofisher) or CellTrace Calcein Red-Orange (Thermofisher).

    Article Title: Antagonistic Inflammatory Phenotypes Dictate Tumor Fate and Response to Immune Checkpoint Blockade
    Article Snippet: Following adhesion for 2h, cells were stained in the wells with 0.5 μM CellTrace Calcein Green (ThermoFisher Scientific) for 20 min. NK cells were stained separately with 0.32 μM CellTrace Calcein Red-Orange (ThermoFisher Scientific) for 20 min.

    Article Title: Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Derivative Assay:

    Article Title: Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
    Article Snippet: On the day of photoactivation, cells were stained with SPACECAT molecules (e.g., calcein NVOC for Fig. ; calcein NVOC, PA-JF549, PA-JF646 for Fig. ) as described in “SPACECAT staining.” For characterization of SPACECAT’s spatial resolution and temporal stability (Figs. and ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Cas9-mediated knockout of Ndrg2 enhances the regenerative potential of dendritic cells for wound healing.
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: Chemical compound, drug , CellTrace Calcein Red-Orange , ThermoFischer , C34851 , (125 nM).

    Article Title: Alpha-synuclein fragments trigger distinct aggregation pathways
    Article Snippet: CellTrace Calcein red-orange (Thermo Fisher Scientific) was used as a cell filling dye prior to fixing with 4% paraformaldehyde (Sigma-Aldrich).

    Article Title: Spatially-Resolved Live Cell Tagging and Isolation Using Protected Photoactivatable Cell Dyes
    Article Snippet: For characterization of SPACECAT’s spatial resolution and temporal stability ( , ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Regulation of sedimentation rate shapes the evolution of multicellularity in a close unicellular relative of animals
    Article Snippet: For cytoplasmic staining of cells in , cells were either stained with CellTrace CFSE Cell Proliferation Kit (Thermofisher) or CellTrace Calcein Red-Orange (Thermofisher).

    Article Title: Antagonistic Inflammatory Phenotypes Dictate Tumor Fate and Response to Immune Checkpoint Blockade
    Article Snippet: Following adhesion for 2h, cells were stained in the wells with 0.5 μM CellTrace Calcein Green (ThermoFisher Scientific) for 20 min. NK cells were stained separately with 0.32 μM CellTrace Calcein Red-Orange (ThermoFisher Scientific) for 20 min.

    Article Title: Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Expressing:

    Article Title: Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
    Article Snippet: On the day of photoactivation, cells were stained with SPACECAT molecules (e.g., calcein NVOC for Fig. ; calcein NVOC, PA-JF549, PA-JF646 for Fig. ) as described in “SPACECAT staining.” For characterization of SPACECAT’s spatial resolution and temporal stability (Figs. and ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Cas9-mediated knockout of Ndrg2 enhances the regenerative potential of dendritic cells for wound healing.
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: Chemical compound, drug , CellTrace Calcein Red-Orange , ThermoFischer , C34851 , (125 nM).

    Article Title: Alpha-synuclein fragments trigger distinct aggregation pathways
    Article Snippet: CellTrace Calcein red-orange (Thermo Fisher Scientific) was used as a cell filling dye prior to fixing with 4% paraformaldehyde (Sigma-Aldrich).

    Article Title: Spatially-Resolved Live Cell Tagging and Isolation Using Protected Photoactivatable Cell Dyes
    Article Snippet: For characterization of SPACECAT’s spatial resolution and temporal stability ( , ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Regulation of sedimentation rate shapes the evolution of multicellularity in a close unicellular relative of animals
    Article Snippet: For cytoplasmic staining of cells in , cells were either stained with CellTrace CFSE Cell Proliferation Kit (Thermofisher) or CellTrace Calcein Red-Orange (Thermofisher).

    Article Title: Antagonistic Inflammatory Phenotypes Dictate Tumor Fate and Response to Immune Checkpoint Blockade
    Article Snippet: Following adhesion for 2h, cells were stained in the wells with 0.5 μM CellTrace Calcein Green (ThermoFisher Scientific) for 20 min. NK cells were stained separately with 0.32 μM CellTrace Calcein Red-Orange (ThermoFisher Scientific) for 20 min.

    Article Title: Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Flow Cytometry:

    Article Title: Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
    Article Snippet: On the day of photoactivation, cells were stained with SPACECAT molecules (e.g., calcein NVOC for Fig. ; calcein NVOC, PA-JF549, PA-JF646 for Fig. ) as described in “SPACECAT staining.” For characterization of SPACECAT’s spatial resolution and temporal stability (Figs. and ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Cas9-mediated knockout of Ndrg2 enhances the regenerative potential of dendritic cells for wound healing.
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: Chemical compound, drug , CellTrace Calcein Red-Orange , ThermoFischer , C34851 , (125 nM).

    Article Title: Alpha-synuclein fragments trigger distinct aggregation pathways
    Article Snippet: CellTrace Calcein red-orange (Thermo Fisher Scientific) was used as a cell filling dye prior to fixing with 4% paraformaldehyde (Sigma-Aldrich).

    Article Title: Spatially-Resolved Live Cell Tagging and Isolation Using Protected Photoactivatable Cell Dyes
    Article Snippet: For characterization of SPACECAT’s spatial resolution and temporal stability ( , ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Regulation of sedimentation rate shapes the evolution of multicellularity in a close unicellular relative of animals
    Article Snippet: For cytoplasmic staining of cells in , cells were either stained with CellTrace CFSE Cell Proliferation Kit (Thermofisher) or CellTrace Calcein Red-Orange (Thermofisher).

    Article Title: Antagonistic Inflammatory Phenotypes Dictate Tumor Fate and Response to Immune Checkpoint Blockade
    Article Snippet: Following adhesion for 2h, cells were stained in the wells with 0.5 μM CellTrace Calcein Green (ThermoFisher Scientific) for 20 min. NK cells were stained separately with 0.32 μM CellTrace Calcein Red-Orange (ThermoFisher Scientific) for 20 min.

    Article Title: Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Co-Culture Assay:

    Article Title: Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
    Article Snippet: On the day of photoactivation, cells were stained with SPACECAT molecules (e.g., calcein NVOC for Fig. ; calcein NVOC, PA-JF549, PA-JF646 for Fig. ) as described in “SPACECAT staining.” For characterization of SPACECAT’s spatial resolution and temporal stability (Figs. and ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Cas9-mediated knockout of Ndrg2 enhances the regenerative potential of dendritic cells for wound healing.
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: Chemical compound, drug , CellTrace Calcein Red-Orange , ThermoFischer , C34851 , (125 nM).

    Article Title: Alpha-synuclein fragments trigger distinct aggregation pathways
    Article Snippet: CellTrace Calcein red-orange (Thermo Fisher Scientific) was used as a cell filling dye prior to fixing with 4% paraformaldehyde (Sigma-Aldrich).

    Article Title: Spatially-Resolved Live Cell Tagging and Isolation Using Protected Photoactivatable Cell Dyes
    Article Snippet: For characterization of SPACECAT’s spatial resolution and temporal stability ( , ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Regulation of sedimentation rate shapes the evolution of multicellularity in a close unicellular relative of animals
    Article Snippet: For cytoplasmic staining of cells in , cells were either stained with CellTrace CFSE Cell Proliferation Kit (Thermofisher) or CellTrace Calcein Red-Orange (Thermofisher).

    Article Title: Antagonistic Inflammatory Phenotypes Dictate Tumor Fate and Response to Immune Checkpoint Blockade
    Article Snippet: Following adhesion for 2h, cells were stained in the wells with 0.5 μM CellTrace Calcein Green (ThermoFisher Scientific) for 20 min. NK cells were stained separately with 0.32 μM CellTrace Calcein Red-Orange (ThermoFisher Scientific) for 20 min.

    Article Title: Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Luminex:

    Article Title: Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
    Article Snippet: On the day of photoactivation, cells were stained with SPACECAT molecules (e.g., calcein NVOC for Fig. ; calcein NVOC, PA-JF549, PA-JF646 for Fig. ) as described in “SPACECAT staining.” For characterization of SPACECAT’s spatial resolution and temporal stability (Figs. and ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Cas9-mediated knockout of Ndrg2 enhances the regenerative potential of dendritic cells for wound healing.
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: Chemical compound, drug , CellTrace Calcein Red-Orange , ThermoFischer , C34851 , (125 nM).

    Article Title: Alpha-synuclein fragments trigger distinct aggregation pathways
    Article Snippet: CellTrace Calcein red-orange (Thermo Fisher Scientific) was used as a cell filling dye prior to fixing with 4% paraformaldehyde (Sigma-Aldrich).

    Article Title: Spatially-Resolved Live Cell Tagging and Isolation Using Protected Photoactivatable Cell Dyes
    Article Snippet: For characterization of SPACECAT’s spatial resolution and temporal stability ( , ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Regulation of sedimentation rate shapes the evolution of multicellularity in a close unicellular relative of animals
    Article Snippet: For cytoplasmic staining of cells in , cells were either stained with CellTrace CFSE Cell Proliferation Kit (Thermofisher) or CellTrace Calcein Red-Orange (Thermofisher).

    Article Title: Antagonistic Inflammatory Phenotypes Dictate Tumor Fate and Response to Immune Checkpoint Blockade
    Article Snippet: Following adhesion for 2h, cells were stained in the wells with 0.5 μM CellTrace Calcein Green (ThermoFisher Scientific) for 20 min. NK cells were stained separately with 0.32 μM CellTrace Calcein Red-Orange (ThermoFisher Scientific) for 20 min.

    Article Title: Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Multiplex Assay:

    Article Title: Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
    Article Snippet: On the day of photoactivation, cells were stained with SPACECAT molecules (e.g., calcein NVOC for Fig. ; calcein NVOC, PA-JF549, PA-JF646 for Fig. ) as described in “SPACECAT staining.” For characterization of SPACECAT’s spatial resolution and temporal stability (Figs. and ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Cas9-mediated knockout of Ndrg2 enhances the regenerative potential of dendritic cells for wound healing.
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: Chemical compound, drug , CellTrace Calcein Red-Orange , ThermoFischer , C34851 , (125 nM).

    Article Title: Alpha-synuclein fragments trigger distinct aggregation pathways
    Article Snippet: CellTrace Calcein red-orange (Thermo Fisher Scientific) was used as a cell filling dye prior to fixing with 4% paraformaldehyde (Sigma-Aldrich).

    Article Title: Spatially-Resolved Live Cell Tagging and Isolation Using Protected Photoactivatable Cell Dyes
    Article Snippet: For characterization of SPACECAT’s spatial resolution and temporal stability ( , ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Regulation of sedimentation rate shapes the evolution of multicellularity in a close unicellular relative of animals
    Article Snippet: For cytoplasmic staining of cells in , cells were either stained with CellTrace CFSE Cell Proliferation Kit (Thermofisher) or CellTrace Calcein Red-Orange (Thermofisher).

    Article Title: Antagonistic Inflammatory Phenotypes Dictate Tumor Fate and Response to Immune Checkpoint Blockade
    Article Snippet: Following adhesion for 2h, cells were stained in the wells with 0.5 μM CellTrace Calcein Green (ThermoFisher Scientific) for 20 min. NK cells were stained separately with 0.32 μM CellTrace Calcein Red-Orange (ThermoFisher Scientific) for 20 min.

    Article Title: Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
    Article Snippet: On the day of photoactivation, cells were stained with SPACECAT molecules (e.g., calcein NVOC for Fig. ; calcein NVOC, PA-JF549, PA-JF646 for Fig. ) as described in “SPACECAT staining.” For characterization of SPACECAT’s spatial resolution and temporal stability (Figs. and ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Cas9-mediated knockout of Ndrg2 enhances the regenerative potential of dendritic cells for wound healing.
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: Chemical compound, drug , CellTrace Calcein Red-Orange , ThermoFischer , C34851 , (125 nM).

    Article Title: Alpha-synuclein fragments trigger distinct aggregation pathways
    Article Snippet: CellTrace Calcein red-orange (Thermo Fisher Scientific) was used as a cell filling dye prior to fixing with 4% paraformaldehyde (Sigma-Aldrich).

    Article Title: Spatially-Resolved Live Cell Tagging and Isolation Using Protected Photoactivatable Cell Dyes
    Article Snippet: For characterization of SPACECAT’s spatial resolution and temporal stability ( , ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Regulation of sedimentation rate shapes the evolution of multicellularity in a close unicellular relative of animals
    Article Snippet: For cytoplasmic staining of cells in , cells were either stained with CellTrace CFSE Cell Proliferation Kit (Thermofisher) or CellTrace Calcein Red-Orange (Thermofisher).

    Article Title: Antagonistic Inflammatory Phenotypes Dictate Tumor Fate and Response to Immune Checkpoint Blockade
    Article Snippet: Following adhesion for 2h, cells were stained in the wells with 0.5 μM CellTrace Calcein Green (ThermoFisher Scientific) for 20 min. NK cells were stained separately with 0.32 μM CellTrace Calcein Red-Orange (ThermoFisher Scientific) for 20 min.

    Article Title: Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Cell Culture:

    Article Title: Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
    Article Snippet: On the day of photoactivation, cells were stained with SPACECAT molecules (e.g., calcein NVOC for Fig. ; calcein NVOC, PA-JF549, PA-JF646 for Fig. ) as described in “SPACECAT staining.” For characterization of SPACECAT’s spatial resolution and temporal stability (Figs. and ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Cas9-mediated knockout of Ndrg2 enhances the regenerative potential of dendritic cells for wound healing.
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: Chemical compound, drug , CellTrace Calcein Red-Orange , ThermoFischer , C34851 , (125 nM).

    Article Title: Alpha-synuclein fragments trigger distinct aggregation pathways
    Article Snippet: CellTrace Calcein red-orange (Thermo Fisher Scientific) was used as a cell filling dye prior to fixing with 4% paraformaldehyde (Sigma-Aldrich).

    Article Title: Spatially-Resolved Live Cell Tagging and Isolation Using Protected Photoactivatable Cell Dyes
    Article Snippet: For characterization of SPACECAT’s spatial resolution and temporal stability ( , ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Regulation of sedimentation rate shapes the evolution of multicellularity in a close unicellular relative of animals
    Article Snippet: For cytoplasmic staining of cells in , cells were either stained with CellTrace CFSE Cell Proliferation Kit (Thermofisher) or CellTrace Calcein Red-Orange (Thermofisher).

    Article Title: Antagonistic Inflammatory Phenotypes Dictate Tumor Fate and Response to Immune Checkpoint Blockade
    Article Snippet: Following adhesion for 2h, cells were stained in the wells with 0.5 μM CellTrace Calcein Green (ThermoFisher Scientific) for 20 min. NK cells were stained separately with 0.32 μM CellTrace Calcein Red-Orange (ThermoFisher Scientific) for 20 min.

    Article Title: Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Confocal Microscopy:

    Article Title: Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
    Article Snippet: On the day of photoactivation, cells were stained with SPACECAT molecules (e.g., calcein NVOC for Fig. ; calcein NVOC, PA-JF549, PA-JF646 for Fig. ) as described in “SPACECAT staining.” For characterization of SPACECAT’s spatial resolution and temporal stability (Figs. and ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Cas9-mediated knockout of Ndrg2 enhances the regenerative potential of dendritic cells for wound healing.
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: Chemical compound, drug , CellTrace Calcein Red-Orange , ThermoFischer , C34851 , (125 nM).

    Article Title: Alpha-synuclein fragments trigger distinct aggregation pathways
    Article Snippet: CellTrace Calcein red-orange (Thermo Fisher Scientific) was used as a cell filling dye prior to fixing with 4% paraformaldehyde (Sigma-Aldrich).

    Article Title: Spatially-Resolved Live Cell Tagging and Isolation Using Protected Photoactivatable Cell Dyes
    Article Snippet: For characterization of SPACECAT’s spatial resolution and temporal stability ( , ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Regulation of sedimentation rate shapes the evolution of multicellularity in a close unicellular relative of animals
    Article Snippet: For cytoplasmic staining of cells in , cells were either stained with CellTrace CFSE Cell Proliferation Kit (Thermofisher) or CellTrace Calcein Red-Orange (Thermofisher).

    Article Title: Antagonistic Inflammatory Phenotypes Dictate Tumor Fate and Response to Immune Checkpoint Blockade
    Article Snippet: Following adhesion for 2h, cells were stained in the wells with 0.5 μM CellTrace Calcein Green (ThermoFisher Scientific) for 20 min. NK cells were stained separately with 0.32 μM CellTrace Calcein Red-Orange (ThermoFisher Scientific) for 20 min.

    Article Title: Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Fluorescence:

    Article Title: Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
    Article Snippet: On the day of photoactivation, cells were stained with SPACECAT molecules (e.g., calcein NVOC for Fig. ; calcein NVOC, PA-JF549, PA-JF646 for Fig. ) as described in “SPACECAT staining.” For characterization of SPACECAT’s spatial resolution and temporal stability (Figs. and ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Cas9-mediated knockout of Ndrg2 enhances the regenerative potential of dendritic cells for wound healing.
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: Chemical compound, drug , CellTrace Calcein Red-Orange , ThermoFischer , C34851 , (125 nM).

    Article Title: Alpha-synuclein fragments trigger distinct aggregation pathways
    Article Snippet: CellTrace Calcein red-orange (Thermo Fisher Scientific) was used as a cell filling dye prior to fixing with 4% paraformaldehyde (Sigma-Aldrich).

    Article Title: Spatially-Resolved Live Cell Tagging and Isolation Using Protected Photoactivatable Cell Dyes
    Article Snippet: For characterization of SPACECAT’s spatial resolution and temporal stability ( , ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Regulation of sedimentation rate shapes the evolution of multicellularity in a close unicellular relative of animals
    Article Snippet: For cytoplasmic staining of cells in , cells were either stained with CellTrace CFSE Cell Proliferation Kit (Thermofisher) or CellTrace Calcein Red-Orange (Thermofisher).

    Article Title: Antagonistic Inflammatory Phenotypes Dictate Tumor Fate and Response to Immune Checkpoint Blockade
    Article Snippet: Following adhesion for 2h, cells were stained in the wells with 0.5 μM CellTrace Calcein Green (ThermoFisher Scientific) for 20 min. NK cells were stained separately with 0.32 μM CellTrace Calcein Red-Orange (ThermoFisher Scientific) for 20 min.

    Article Title: Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Selection:

    Article Title: Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
    Article Snippet: On the day of photoactivation, cells were stained with SPACECAT molecules (e.g., calcein NVOC for Fig. ; calcein NVOC, PA-JF549, PA-JF646 for Fig. ) as described in “SPACECAT staining.” For characterization of SPACECAT’s spatial resolution and temporal stability (Figs. and ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Cas9-mediated knockout of Ndrg2 enhances the regenerative potential of dendritic cells for wound healing.
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: Chemical compound, drug , CellTrace Calcein Red-Orange , ThermoFischer , C34851 , (125 nM).

    Article Title: Alpha-synuclein fragments trigger distinct aggregation pathways
    Article Snippet: CellTrace Calcein red-orange (Thermo Fisher Scientific) was used as a cell filling dye prior to fixing with 4% paraformaldehyde (Sigma-Aldrich).

    Article Title: Spatially-Resolved Live Cell Tagging and Isolation Using Protected Photoactivatable Cell Dyes
    Article Snippet: For characterization of SPACECAT’s spatial resolution and temporal stability ( , ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Regulation of sedimentation rate shapes the evolution of multicellularity in a close unicellular relative of animals
    Article Snippet: For cytoplasmic staining of cells in , cells were either stained with CellTrace CFSE Cell Proliferation Kit (Thermofisher) or CellTrace Calcein Red-Orange (Thermofisher).

    Article Title: Antagonistic Inflammatory Phenotypes Dictate Tumor Fate and Response to Immune Checkpoint Blockade
    Article Snippet: Following adhesion for 2h, cells were stained in the wells with 0.5 μM CellTrace Calcein Green (ThermoFisher Scientific) for 20 min. NK cells were stained separately with 0.32 μM CellTrace Calcein Red-Orange (ThermoFisher Scientific) for 20 min.

    Article Title: Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Isolation:

    Article Title: Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
    Article Snippet: On the day of photoactivation, cells were stained with SPACECAT molecules (e.g., calcein NVOC for Fig. ; calcein NVOC, PA-JF549, PA-JF646 for Fig. ) as described in “SPACECAT staining.” For characterization of SPACECAT’s spatial resolution and temporal stability (Figs. and ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Cas9-mediated knockout of Ndrg2 enhances the regenerative potential of dendritic cells for wound healing.
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: Chemical compound, drug , CellTrace Calcein Red-Orange , ThermoFischer , C34851 , (125 nM).

    Article Title: Alpha-synuclein fragments trigger distinct aggregation pathways
    Article Snippet: CellTrace Calcein red-orange (Thermo Fisher Scientific) was used as a cell filling dye prior to fixing with 4% paraformaldehyde (Sigma-Aldrich).

    Article Title: Spatially-Resolved Live Cell Tagging and Isolation Using Protected Photoactivatable Cell Dyes
    Article Snippet: For characterization of SPACECAT’s spatial resolution and temporal stability ( , ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Regulation of sedimentation rate shapes the evolution of multicellularity in a close unicellular relative of animals
    Article Snippet: For cytoplasmic staining of cells in , cells were either stained with CellTrace CFSE Cell Proliferation Kit (Thermofisher) or CellTrace Calcein Red-Orange (Thermofisher).

    Article Title: Antagonistic Inflammatory Phenotypes Dictate Tumor Fate and Response to Immune Checkpoint Blockade
    Article Snippet: Following adhesion for 2h, cells were stained in the wells with 0.5 μM CellTrace Calcein Green (ThermoFisher Scientific) for 20 min. NK cells were stained separately with 0.32 μM CellTrace Calcein Red-Orange (ThermoFisher Scientific) for 20 min.

    Article Title: Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Marker:

    Article Title: Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
    Article Snippet: On the day of photoactivation, cells were stained with SPACECAT molecules (e.g., calcein NVOC for Fig. ; calcein NVOC, PA-JF549, PA-JF646 for Fig. ) as described in “SPACECAT staining.” For characterization of SPACECAT’s spatial resolution and temporal stability (Figs. and ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Cas9-mediated knockout of Ndrg2 enhances the regenerative potential of dendritic cells for wound healing.
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: Chemical compound, drug , CellTrace Calcein Red-Orange , ThermoFischer , C34851 , (125 nM).

    Article Title: Alpha-synuclein fragments trigger distinct aggregation pathways
    Article Snippet: CellTrace Calcein red-orange (Thermo Fisher Scientific) was used as a cell filling dye prior to fixing with 4% paraformaldehyde (Sigma-Aldrich).

    Article Title: Spatially-Resolved Live Cell Tagging and Isolation Using Protected Photoactivatable Cell Dyes
    Article Snippet: For characterization of SPACECAT’s spatial resolution and temporal stability ( , ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Regulation of sedimentation rate shapes the evolution of multicellularity in a close unicellular relative of animals
    Article Snippet: For cytoplasmic staining of cells in , cells were either stained with CellTrace CFSE Cell Proliferation Kit (Thermofisher) or CellTrace Calcein Red-Orange (Thermofisher).

    Article Title: Antagonistic Inflammatory Phenotypes Dictate Tumor Fate and Response to Immune Checkpoint Blockade
    Article Snippet: Following adhesion for 2h, cells were stained in the wells with 0.5 μM CellTrace Calcein Green (ThermoFisher Scientific) for 20 min. NK cells were stained separately with 0.32 μM CellTrace Calcein Red-Orange (ThermoFisher Scientific) for 20 min.

    Article Title: Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Migration:

    Article Title: Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
    Article Snippet: On the day of photoactivation, cells were stained with SPACECAT molecules (e.g., calcein NVOC for Fig. ; calcein NVOC, PA-JF549, PA-JF646 for Fig. ) as described in “SPACECAT staining.” For characterization of SPACECAT’s spatial resolution and temporal stability (Figs. and ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Cas9-mediated knockout of Ndrg2 enhances the regenerative potential of dendritic cells for wound healing.
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: Chemical compound, drug , CellTrace Calcein Red-Orange , ThermoFischer , C34851 , (125 nM).

    Article Title: Alpha-synuclein fragments trigger distinct aggregation pathways
    Article Snippet: CellTrace Calcein red-orange (Thermo Fisher Scientific) was used as a cell filling dye prior to fixing with 4% paraformaldehyde (Sigma-Aldrich).

    Article Title: Spatially-Resolved Live Cell Tagging and Isolation Using Protected Photoactivatable Cell Dyes
    Article Snippet: For characterization of SPACECAT’s spatial resolution and temporal stability ( , ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Regulation of sedimentation rate shapes the evolution of multicellularity in a close unicellular relative of animals
    Article Snippet: For cytoplasmic staining of cells in , cells were either stained with CellTrace CFSE Cell Proliferation Kit (Thermofisher) or CellTrace Calcein Red-Orange (Thermofisher).

    Article Title: Antagonistic Inflammatory Phenotypes Dictate Tumor Fate and Response to Immune Checkpoint Blockade
    Article Snippet: Following adhesion for 2h, cells were stained in the wells with 0.5 μM CellTrace Calcein Green (ThermoFisher Scientific) for 20 min. NK cells were stained separately with 0.32 μM CellTrace Calcein Red-Orange (ThermoFisher Scientific) for 20 min.

    Article Title: Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Recombinant:

    Article Title: Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
    Article Snippet: On the day of photoactivation, cells were stained with SPACECAT molecules (e.g., calcein NVOC for Fig. ; calcein NVOC, PA-JF549, PA-JF646 for Fig. ) as described in “SPACECAT staining.” For characterization of SPACECAT’s spatial resolution and temporal stability (Figs. and ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Cas9-mediated knockout of Ndrg2 enhances the regenerative potential of dendritic cells for wound healing.
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: Chemical compound, drug , CellTrace Calcein Red-Orange , ThermoFischer , C34851 , (125 nM).

    Article Title: Alpha-synuclein fragments trigger distinct aggregation pathways
    Article Snippet: CellTrace Calcein red-orange (Thermo Fisher Scientific) was used as a cell filling dye prior to fixing with 4% paraformaldehyde (Sigma-Aldrich).

    Article Title: Spatially-Resolved Live Cell Tagging and Isolation Using Protected Photoactivatable Cell Dyes
    Article Snippet: For characterization of SPACECAT’s spatial resolution and temporal stability ( , ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Regulation of sedimentation rate shapes the evolution of multicellularity in a close unicellular relative of animals
    Article Snippet: For cytoplasmic staining of cells in , cells were either stained with CellTrace CFSE Cell Proliferation Kit (Thermofisher) or CellTrace Calcein Red-Orange (Thermofisher).

    Article Title: Antagonistic Inflammatory Phenotypes Dictate Tumor Fate and Response to Immune Checkpoint Blockade
    Article Snippet: Following adhesion for 2h, cells were stained in the wells with 0.5 μM CellTrace Calcein Green (ThermoFisher Scientific) for 20 min. NK cells were stained separately with 0.32 μM CellTrace Calcein Red-Orange (ThermoFisher Scientific) for 20 min.

    Article Title: Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Cell Isolation:

    Article Title: Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
    Article Snippet: On the day of photoactivation, cells were stained with SPACECAT molecules (e.g., calcein NVOC for Fig. ; calcein NVOC, PA-JF549, PA-JF646 for Fig. ) as described in “SPACECAT staining.” For characterization of SPACECAT’s spatial resolution and temporal stability (Figs. and ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Cas9-mediated knockout of Ndrg2 enhances the regenerative potential of dendritic cells for wound healing.
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: Chemical compound, drug , CellTrace Calcein Red-Orange , ThermoFischer , C34851 , (125 nM).

    Article Title: Alpha-synuclein fragments trigger distinct aggregation pathways
    Article Snippet: CellTrace Calcein red-orange (Thermo Fisher Scientific) was used as a cell filling dye prior to fixing with 4% paraformaldehyde (Sigma-Aldrich).

    Article Title: Spatially-Resolved Live Cell Tagging and Isolation Using Protected Photoactivatable Cell Dyes
    Article Snippet: For characterization of SPACECAT’s spatial resolution and temporal stability ( , ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Regulation of sedimentation rate shapes the evolution of multicellularity in a close unicellular relative of animals
    Article Snippet: For cytoplasmic staining of cells in , cells were either stained with CellTrace CFSE Cell Proliferation Kit (Thermofisher) or CellTrace Calcein Red-Orange (Thermofisher).

    Article Title: Antagonistic Inflammatory Phenotypes Dictate Tumor Fate and Response to Immune Checkpoint Blockade
    Article Snippet: Following adhesion for 2h, cells were stained in the wells with 0.5 μM CellTrace Calcein Green (ThermoFisher Scientific) for 20 min. NK cells were stained separately with 0.32 μM CellTrace Calcein Red-Orange (ThermoFisher Scientific) for 20 min.

    Article Title: Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Activation Assay:

    Article Title: Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
    Article Snippet: On the day of photoactivation, cells were stained with SPACECAT molecules (e.g., calcein NVOC for Fig. ; calcein NVOC, PA-JF549, PA-JF646 for Fig. ) as described in “SPACECAT staining.” For characterization of SPACECAT’s spatial resolution and temporal stability (Figs. and ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Cas9-mediated knockout of Ndrg2 enhances the regenerative potential of dendritic cells for wound healing.
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: Chemical compound, drug , CellTrace Calcein Red-Orange , ThermoFischer , C34851 , (125 nM).

    Article Title: Alpha-synuclein fragments trigger distinct aggregation pathways
    Article Snippet: CellTrace Calcein red-orange (Thermo Fisher Scientific) was used as a cell filling dye prior to fixing with 4% paraformaldehyde (Sigma-Aldrich).

    Article Title: Spatially-Resolved Live Cell Tagging and Isolation Using Protected Photoactivatable Cell Dyes
    Article Snippet: For characterization of SPACECAT’s spatial resolution and temporal stability ( , ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Regulation of sedimentation rate shapes the evolution of multicellularity in a close unicellular relative of animals
    Article Snippet: For cytoplasmic staining of cells in , cells were either stained with CellTrace CFSE Cell Proliferation Kit (Thermofisher) or CellTrace Calcein Red-Orange (Thermofisher).

    Article Title: Antagonistic Inflammatory Phenotypes Dictate Tumor Fate and Response to Immune Checkpoint Blockade
    Article Snippet: Following adhesion for 2h, cells were stained in the wells with 0.5 μM CellTrace Calcein Green (ThermoFisher Scientific) for 20 min. NK cells were stained separately with 0.32 μM CellTrace Calcein Red-Orange (ThermoFisher Scientific) for 20 min.

    Article Title: Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Software:

    Article Title: Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
    Article Snippet: On the day of photoactivation, cells were stained with SPACECAT molecules (e.g., calcein NVOC for Fig. ; calcein NVOC, PA-JF549, PA-JF646 for Fig. ) as described in “SPACECAT staining.” For characterization of SPACECAT’s spatial resolution and temporal stability (Figs. and ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Cas9-mediated knockout of Ndrg2 enhances the regenerative potential of dendritic cells for wound healing.
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: Chemical compound, drug , CellTrace Calcein Red-Orange , ThermoFischer , C34851 , (125 nM).

    Article Title: Alpha-synuclein fragments trigger distinct aggregation pathways
    Article Snippet: CellTrace Calcein red-orange (Thermo Fisher Scientific) was used as a cell filling dye prior to fixing with 4% paraformaldehyde (Sigma-Aldrich).

    Article Title: Spatially-Resolved Live Cell Tagging and Isolation Using Protected Photoactivatable Cell Dyes
    Article Snippet: For characterization of SPACECAT’s spatial resolution and temporal stability ( , ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Regulation of sedimentation rate shapes the evolution of multicellularity in a close unicellular relative of animals
    Article Snippet: For cytoplasmic staining of cells in , cells were either stained with CellTrace CFSE Cell Proliferation Kit (Thermofisher) or CellTrace Calcein Red-Orange (Thermofisher).

    Article Title: Antagonistic Inflammatory Phenotypes Dictate Tumor Fate and Response to Immune Checkpoint Blockade
    Article Snippet: Following adhesion for 2h, cells were stained in the wells with 0.5 μM CellTrace Calcein Green (ThermoFisher Scientific) for 20 min. NK cells were stained separately with 0.32 μM CellTrace Calcein Red-Orange (ThermoFisher Scientific) for 20 min.

    Article Title: Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Control:

    Article Title: Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
    Article Snippet: On the day of photoactivation, cells were stained with SPACECAT molecules (e.g., calcein NVOC for Fig. ; calcein NVOC, PA-JF549, PA-JF646 for Fig. ) as described in “SPACECAT staining.” For characterization of SPACECAT’s spatial resolution and temporal stability (Figs. and ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Cas9-mediated knockout of Ndrg2 enhances the regenerative potential of dendritic cells for wound healing.
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: Chemical compound, drug , CellTrace Calcein Red-Orange , ThermoFischer , C34851 , (125 nM).

    Article Title: Alpha-synuclein fragments trigger distinct aggregation pathways
    Article Snippet: CellTrace Calcein red-orange (Thermo Fisher Scientific) was used as a cell filling dye prior to fixing with 4% paraformaldehyde (Sigma-Aldrich).

    Article Title: Spatially-Resolved Live Cell Tagging and Isolation Using Protected Photoactivatable Cell Dyes
    Article Snippet: For characterization of SPACECAT’s spatial resolution and temporal stability ( , ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Regulation of sedimentation rate shapes the evolution of multicellularity in a close unicellular relative of animals
    Article Snippet: For cytoplasmic staining of cells in , cells were either stained with CellTrace CFSE Cell Proliferation Kit (Thermofisher) or CellTrace Calcein Red-Orange (Thermofisher).

    Article Title: Antagonistic Inflammatory Phenotypes Dictate Tumor Fate and Response to Immune Checkpoint Blockade
    Article Snippet: Following adhesion for 2h, cells were stained in the wells with 0.5 μM CellTrace Calcein Green (ThermoFisher Scientific) for 20 min. NK cells were stained separately with 0.32 μM CellTrace Calcein Red-Orange (ThermoFisher Scientific) for 20 min.

    Article Title: Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Purification:

    Article Title: Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
    Article Snippet: On the day of photoactivation, cells were stained with SPACECAT molecules (e.g., calcein NVOC for Fig. ; calcein NVOC, PA-JF549, PA-JF646 for Fig. ) as described in “SPACECAT staining.” For characterization of SPACECAT’s spatial resolution and temporal stability (Figs. and ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Cas9-mediated knockout of Ndrg2 enhances the regenerative potential of dendritic cells for wound healing.
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: Chemical compound, drug , CellTrace Calcein Red-Orange , ThermoFischer , C34851 , (125 nM).

    Article Title: Alpha-synuclein fragments trigger distinct aggregation pathways
    Article Snippet: CellTrace Calcein red-orange (Thermo Fisher Scientific) was used as a cell filling dye prior to fixing with 4% paraformaldehyde (Sigma-Aldrich).

    Article Title: Spatially-Resolved Live Cell Tagging and Isolation Using Protected Photoactivatable Cell Dyes
    Article Snippet: For characterization of SPACECAT’s spatial resolution and temporal stability ( , ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Regulation of sedimentation rate shapes the evolution of multicellularity in a close unicellular relative of animals
    Article Snippet: For cytoplasmic staining of cells in , cells were either stained with CellTrace CFSE Cell Proliferation Kit (Thermofisher) or CellTrace Calcein Red-Orange (Thermofisher).

    Article Title: Antagonistic Inflammatory Phenotypes Dictate Tumor Fate and Response to Immune Checkpoint Blockade
    Article Snippet: Following adhesion for 2h, cells were stained in the wells with 0.5 μM CellTrace Calcein Green (ThermoFisher Scientific) for 20 min. NK cells were stained separately with 0.32 μM CellTrace Calcein Red-Orange (ThermoFisher Scientific) for 20 min.

    Article Title: Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Affinity Purification:

    Article Title: Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
    Article Snippet: On the day of photoactivation, cells were stained with SPACECAT molecules (e.g., calcein NVOC for Fig. ; calcein NVOC, PA-JF549, PA-JF646 for Fig. ) as described in “SPACECAT staining.” For characterization of SPACECAT’s spatial resolution and temporal stability (Figs. and ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Cas9-mediated knockout of Ndrg2 enhances the regenerative potential of dendritic cells for wound healing.
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: Chemical compound, drug , CellTrace Calcein Red-Orange , ThermoFischer , C34851 , (125 nM).

    Article Title: Alpha-synuclein fragments trigger distinct aggregation pathways
    Article Snippet: CellTrace Calcein red-orange (Thermo Fisher Scientific) was used as a cell filling dye prior to fixing with 4% paraformaldehyde (Sigma-Aldrich).

    Article Title: Spatially-Resolved Live Cell Tagging and Isolation Using Protected Photoactivatable Cell Dyes
    Article Snippet: For characterization of SPACECAT’s spatial resolution and temporal stability ( , ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Regulation of sedimentation rate shapes the evolution of multicellularity in a close unicellular relative of animals
    Article Snippet: For cytoplasmic staining of cells in , cells were either stained with CellTrace CFSE Cell Proliferation Kit (Thermofisher) or CellTrace Calcein Red-Orange (Thermofisher).

    Article Title: Antagonistic Inflammatory Phenotypes Dictate Tumor Fate and Response to Immune Checkpoint Blockade
    Article Snippet: Following adhesion for 2h, cells were stained in the wells with 0.5 μM CellTrace Calcein Green (ThermoFisher Scientific) for 20 min. NK cells were stained separately with 0.32 μM CellTrace Calcein Red-Orange (ThermoFisher Scientific) for 20 min.

    Article Title: Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Lysis:

    Article Title: Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
    Article Snippet: On the day of photoactivation, cells were stained with SPACECAT molecules (e.g., calcein NVOC for Fig. ; calcein NVOC, PA-JF549, PA-JF646 for Fig. ) as described in “SPACECAT staining.” For characterization of SPACECAT’s spatial resolution and temporal stability (Figs. and ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Cas9-mediated knockout of Ndrg2 enhances the regenerative potential of dendritic cells for wound healing.
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: Chemical compound, drug , CellTrace Calcein Red-Orange , ThermoFischer , C34851 , (125 nM).

    Article Title: Alpha-synuclein fragments trigger distinct aggregation pathways
    Article Snippet: CellTrace Calcein red-orange (Thermo Fisher Scientific) was used as a cell filling dye prior to fixing with 4% paraformaldehyde (Sigma-Aldrich).

    Article Title: Spatially-Resolved Live Cell Tagging and Isolation Using Protected Photoactivatable Cell Dyes
    Article Snippet: For characterization of SPACECAT’s spatial resolution and temporal stability ( , ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Regulation of sedimentation rate shapes the evolution of multicellularity in a close unicellular relative of animals
    Article Snippet: For cytoplasmic staining of cells in , cells were either stained with CellTrace CFSE Cell Proliferation Kit (Thermofisher) or CellTrace Calcein Red-Orange (Thermofisher).

    Article Title: Antagonistic Inflammatory Phenotypes Dictate Tumor Fate and Response to Immune Checkpoint Blockade
    Article Snippet: Following adhesion for 2h, cells were stained in the wells with 0.5 μM CellTrace Calcein Green (ThermoFisher Scientific) for 20 min. NK cells were stained separately with 0.32 μM CellTrace Calcein Red-Orange (ThermoFisher Scientific) for 20 min.

    Article Title: Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Library Quantification:

    Article Title: Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
    Article Snippet: On the day of photoactivation, cells were stained with SPACECAT molecules (e.g., calcein NVOC for Fig. ; calcein NVOC, PA-JF549, PA-JF646 for Fig. ) as described in “SPACECAT staining.” For characterization of SPACECAT’s spatial resolution and temporal stability (Figs. and ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Cas9-mediated knockout of Ndrg2 enhances the regenerative potential of dendritic cells for wound healing.
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: Chemical compound, drug , CellTrace Calcein Red-Orange , ThermoFischer , C34851 , (125 nM).

    Article Title: Alpha-synuclein fragments trigger distinct aggregation pathways
    Article Snippet: CellTrace Calcein red-orange (Thermo Fisher Scientific) was used as a cell filling dye prior to fixing with 4% paraformaldehyde (Sigma-Aldrich).

    Article Title: Spatially-Resolved Live Cell Tagging and Isolation Using Protected Photoactivatable Cell Dyes
    Article Snippet: For characterization of SPACECAT’s spatial resolution and temporal stability ( , ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Regulation of sedimentation rate shapes the evolution of multicellularity in a close unicellular relative of animals
    Article Snippet: For cytoplasmic staining of cells in , cells were either stained with CellTrace CFSE Cell Proliferation Kit (Thermofisher) or CellTrace Calcein Red-Orange (Thermofisher).

    Article Title: Antagonistic Inflammatory Phenotypes Dictate Tumor Fate and Response to Immune Checkpoint Blockade
    Article Snippet: Following adhesion for 2h, cells were stained in the wells with 0.5 μM CellTrace Calcein Green (ThermoFisher Scientific) for 20 min. NK cells were stained separately with 0.32 μM CellTrace Calcein Red-Orange (ThermoFisher Scientific) for 20 min.

    Article Title: Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Illumina Sequencing:

    Article Title: Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
    Article Snippet: On the day of photoactivation, cells were stained with SPACECAT molecules (e.g., calcein NVOC for Fig. ; calcein NVOC, PA-JF549, PA-JF646 for Fig. ) as described in “SPACECAT staining.” For characterization of SPACECAT’s spatial resolution and temporal stability (Figs. and ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Cas9-mediated knockout of Ndrg2 enhances the regenerative potential of dendritic cells for wound healing.
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: Chemical compound, drug , CellTrace Calcein Red-Orange , ThermoFischer , C34851 , (125 nM).

    Article Title: Alpha-synuclein fragments trigger distinct aggregation pathways
    Article Snippet: CellTrace Calcein red-orange (Thermo Fisher Scientific) was used as a cell filling dye prior to fixing with 4% paraformaldehyde (Sigma-Aldrich).

    Article Title: Spatially-Resolved Live Cell Tagging and Isolation Using Protected Photoactivatable Cell Dyes
    Article Snippet: For characterization of SPACECAT’s spatial resolution and temporal stability ( , ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Regulation of sedimentation rate shapes the evolution of multicellularity in a close unicellular relative of animals
    Article Snippet: For cytoplasmic staining of cells in , cells were either stained with CellTrace CFSE Cell Proliferation Kit (Thermofisher) or CellTrace Calcein Red-Orange (Thermofisher).

    Article Title: Antagonistic Inflammatory Phenotypes Dictate Tumor Fate and Response to Immune Checkpoint Blockade
    Article Snippet: Following adhesion for 2h, cells were stained in the wells with 0.5 μM CellTrace Calcein Green (ThermoFisher Scientific) for 20 min. NK cells were stained separately with 0.32 μM CellTrace Calcein Red-Orange (ThermoFisher Scientific) for 20 min.

    Article Title: Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Irradiation:

    Article Title: Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
    Article Snippet: On the day of photoactivation, cells were stained with SPACECAT molecules (e.g., calcein NVOC for Fig. ; calcein NVOC, PA-JF549, PA-JF646 for Fig. ) as described in “SPACECAT staining.” For characterization of SPACECAT’s spatial resolution and temporal stability (Figs. and ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Cas9-mediated knockout of Ndrg2 enhances the regenerative potential of dendritic cells for wound healing.
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: Chemical compound, drug , CellTrace Calcein Red-Orange , ThermoFischer , C34851 , (125 nM).

    Article Title: Alpha-synuclein fragments trigger distinct aggregation pathways
    Article Snippet: CellTrace Calcein red-orange (Thermo Fisher Scientific) was used as a cell filling dye prior to fixing with 4% paraformaldehyde (Sigma-Aldrich).

    Article Title: Spatially-Resolved Live Cell Tagging and Isolation Using Protected Photoactivatable Cell Dyes
    Article Snippet: For characterization of SPACECAT’s spatial resolution and temporal stability ( , ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Regulation of sedimentation rate shapes the evolution of multicellularity in a close unicellular relative of animals
    Article Snippet: For cytoplasmic staining of cells in , cells were either stained with CellTrace CFSE Cell Proliferation Kit (Thermofisher) or CellTrace Calcein Red-Orange (Thermofisher).

    Article Title: Antagonistic Inflammatory Phenotypes Dictate Tumor Fate and Response to Immune Checkpoint Blockade
    Article Snippet: Following adhesion for 2h, cells were stained in the wells with 0.5 μM CellTrace Calcein Green (ThermoFisher Scientific) for 20 min. NK cells were stained separately with 0.32 μM CellTrace Calcein Red-Orange (ThermoFisher Scientific) for 20 min.

    Article Title: Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Imaging:

    Article Title: Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
    Article Snippet: On the day of photoactivation, cells were stained with SPACECAT molecules (e.g., calcein NVOC for Fig. ; calcein NVOC, PA-JF549, PA-JF646 for Fig. ) as described in “SPACECAT staining.” For characterization of SPACECAT’s spatial resolution and temporal stability (Figs. and ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Cas9-mediated knockout of Ndrg2 enhances the regenerative potential of dendritic cells for wound healing.
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: Chemical compound, drug , CellTrace Calcein Red-Orange , ThermoFischer , C34851 , (125 nM).

    Article Title: Alpha-synuclein fragments trigger distinct aggregation pathways
    Article Snippet: CellTrace Calcein red-orange (Thermo Fisher Scientific) was used as a cell filling dye prior to fixing with 4% paraformaldehyde (Sigma-Aldrich).

    Article Title: Spatially-Resolved Live Cell Tagging and Isolation Using Protected Photoactivatable Cell Dyes
    Article Snippet: For characterization of SPACECAT’s spatial resolution and temporal stability ( , ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Regulation of sedimentation rate shapes the evolution of multicellularity in a close unicellular relative of animals
    Article Snippet: For cytoplasmic staining of cells in , cells were either stained with CellTrace CFSE Cell Proliferation Kit (Thermofisher) or CellTrace Calcein Red-Orange (Thermofisher).

    Article Title: Antagonistic Inflammatory Phenotypes Dictate Tumor Fate and Response to Immune Checkpoint Blockade
    Article Snippet: Following adhesion for 2h, cells were stained in the wells with 0.5 μM CellTrace Calcein Green (ThermoFisher Scientific) for 20 min. NK cells were stained separately with 0.32 μM CellTrace Calcein Red-Orange (ThermoFisher Scientific) for 20 min.

    Article Title: Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Labeling:

    Article Title: Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
    Article Snippet: On the day of photoactivation, cells were stained with SPACECAT molecules (e.g., calcein NVOC for Fig. ; calcein NVOC, PA-JF549, PA-JF646 for Fig. ) as described in “SPACECAT staining.” For characterization of SPACECAT’s spatial resolution and temporal stability (Figs. and ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Cas9-mediated knockout of Ndrg2 enhances the regenerative potential of dendritic cells for wound healing.
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.

    Article Title: Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment
    Article Snippet: Chemical compound, drug , CellTrace Calcein Red-Orange , ThermoFischer , C34851 , (125 nM).

    Article Title: Alpha-synuclein fragments trigger distinct aggregation pathways
    Article Snippet: CellTrace Calcein red-orange (Thermo Fisher Scientific) was used as a cell filling dye prior to fixing with 4% paraformaldehyde (Sigma-Aldrich).

    Article Title: Spatially-Resolved Live Cell Tagging and Isolation Using Protected Photoactivatable Cell Dyes
    Article Snippet: For characterization of SPACECAT’s spatial resolution and temporal stability ( , ), cells were also stained with CellTrace Calcein Red-Orange (ThermoFisher) and Hoechst (ThermoFisher) at 1:10 4 and 1:10 5 concentrations in Hank’s balance salt Solution (Life Technologies), respectively.

    Article Title: Regulation of sedimentation rate shapes the evolution of multicellularity in a close unicellular relative of animals
    Article Snippet: For cytoplasmic staining of cells in , cells were either stained with CellTrace CFSE Cell Proliferation Kit (Thermofisher) or CellTrace Calcein Red-Orange (Thermofisher).

    Article Title: Antagonistic Inflammatory Phenotypes Dictate Tumor Fate and Response to Immune Checkpoint Blockade
    Article Snippet: Following adhesion for 2h, cells were stained in the wells with 0.5 μM CellTrace Calcein Green (ThermoFisher Scientific) for 20 min. NK cells were stained separately with 0.32 μM CellTrace Calcein Red-Orange (ThermoFisher Scientific) for 20 min.

    Article Title: Cas9-Mediated Knockout of Ndrg2 Enhances the Regenerative Potential of Dendritic Cells for Wound Healing
    Article Snippet: For co-culture assays, DCs were stained using CellTrace Calcein Red-Orange (ThermoFisher) and added to the EC cultures at a 1:1 ratio.



    Similar Products

    90
    Thermo Fisher celltrace calcein red-orange, am
    Celltrace Calcein Red Orange, Am, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/celltrace+calcein+red-orange/celltrace+calcein+red+orange+am/pm40020762-299-0-14
    Average 90 stars, based on 1 article reviews
    celltrace calcein red-orange, am - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher celltrace™ calcein red-orange
    Celltrace™ Calcein Red Orange, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/celltrace+calcein+red-orange/bio_rxiv__2024__12__13__628384-297-29-28
    Average 90 stars, based on 1 article reviews
    celltrace™ calcein red-orange - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher celltrace calcein red-orange am
    Celltrace Calcein Red Orange Am, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/celltrace+calcein+red-orange/celltrace+calcein+red+orange+am/pm39120083-129-13-22
    Average 90 stars, based on 1 article reviews
    celltrace calcein red-orange am - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    86
    Thermo Fisher celltrace calcein red orange
    ( A ) Heatmap of TJAP-coding mRNAs in human primary monocytes and the THP-1 cell line based on existing RNAseq databases (see Methods). ( B ) siRNA screen on monocytic TJAP proteins to define their role in transmigration. The scheme represents the experimental setup of a transmigration assay with monocytes crossing a confluent monolayer of brain endothelial cells (hCMEC/D3). TJAPs were selected based on the heatmap shown in A. THP-1 cells were electroporated with the indicated siRNAs and added to the top chamber for 17 h (overnight). The data is presented as percentage of transmigrated THP-1 cells normalized to the siRNA control (black line). The graph shows n = 3 independent experiments performed at least in two technical replicates. ( C ) Percentage of transmigrated OCLN KO THP-1 cells rescued either with EGFP-CAAX, EGFP-OCLN, or EGFP-OCLN-ΔC across hCMEC/D3 monolayer. The data were obtained from n = 3 individual experiments performed in two technical replicates normalized to EGFP-CAAX of each experiment. Each symbol’s color corresponds to an individual experiment. Red dashes represent the mean of experiments combined together. ( D ) Percentage of adherent primary monocytes expressing EGFP-CAAX, EGFP-OCLN, or EGFP-OCLN-ΔC on fibronectin substrate measured by flow cytometry. Adhesion time is 30 min. The data were obtained from n = 4 monocyte donors. Data were normalized to the average of EGFP-CAAX obtained from each experiment. Each symbol’s color corresponds to an individual monocyte donor. ( E ) Percentage of adherent primary monocytes expressing EGFP-CAAX, EGFP-OCLN, or EGFP-OCLN-ΔC on hCMEC/D3 monolayer measured by flow cytometry. Adhesion time is 30 min. The data represents the mean values obtained from n = 7 monocyte donors. Data were normalized to the average of EGFP-CAAX obtained from each experiment. Each symbol’s color corresponds to an individual monocyte donor. Error bars are SEM. ( F ) Percentage of transmigrated primary monocytes expressing EGFP-CAAX, EGFP-OCLN, or EGFP-OCLN-ΔC across hCMEC/D3 monolayer. The data were obtained from n = 6 monocyte donors. Data were normalized to the average of EGFP-CAAX obtained from each experiment. Each symbol’s color corresponds to an individual monocyte donor. ( G , H ) 3D time-lapse spinning disk confocal microscopy of transmigrating monocytes. Fluorescence intensity scale is shown on the side of each panel. EGFP is represented using the “red Fire” color-coding, and endothelial cells are stained with a <t>CellTrace</t> marker (cyan) added prior to the addition of the monocytes. Timescales are shown is the bottom right corner and correspond to the time after which imaging acquisition started. ( G ) Imaging of a primary monocyte transduced with EGFP-OCLN on hCMEC/D3 monolayer. Images were taken every 10 min. Scale bar: 10 μm. Full video can be found in Movie . ( H ) Imaging of a THP-1 cell transduced with EGFP-OCLN on hCMEC/D3 monolayer (not stained). Images were taken every 10 min. Scale bar: 8 μm. Full video can be found in Movie . The white square highlights OCLN accumulation at the potential interface between hCMEC/D3 cells and the THP-1 cell. (i) Enlarged image from the white square and a 90° flip showing the accumulation of OCLN. Scale bar: 0.7 µm. Data information: In ( B , C , F ), data are presented as mean ± SEM. In ( D , E ), error bars represent SEM. Two-tailed Student’s t-test p value < 0.05 (*), < 0.01 (**) or non-significant (ns). .
    Celltrace Calcein Red Orange, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/celltrace+calcein+red-orange/pmc11315906-111-0-4
    Average 86 stars, based on 1 article reviews
    celltrace calcein red orange - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    90
    Thermo Fisher celltrace calcein red-orange am c34851
    ( A ) Heatmap of TJAP-coding mRNAs in human primary monocytes and the THP-1 cell line based on existing RNAseq databases (see Methods). ( B ) siRNA screen on monocytic TJAP proteins to define their role in transmigration. The scheme represents the experimental setup of a transmigration assay with monocytes crossing a confluent monolayer of brain endothelial cells (hCMEC/D3). TJAPs were selected based on the heatmap shown in A. THP-1 cells were electroporated with the indicated siRNAs and added to the top chamber for 17 h (overnight). The data is presented as percentage of transmigrated THP-1 cells normalized to the siRNA control (black line). The graph shows n = 3 independent experiments performed at least in two technical replicates. ( C ) Percentage of transmigrated OCLN KO THP-1 cells rescued either with EGFP-CAAX, EGFP-OCLN, or EGFP-OCLN-ΔC across hCMEC/D3 monolayer. The data were obtained from n = 3 individual experiments performed in two technical replicates normalized to EGFP-CAAX of each experiment. Each symbol’s color corresponds to an individual experiment. Red dashes represent the mean of experiments combined together. ( D ) Percentage of adherent primary monocytes expressing EGFP-CAAX, EGFP-OCLN, or EGFP-OCLN-ΔC on fibronectin substrate measured by flow cytometry. Adhesion time is 30 min. The data were obtained from n = 4 monocyte donors. Data were normalized to the average of EGFP-CAAX obtained from each experiment. Each symbol’s color corresponds to an individual monocyte donor. ( E ) Percentage of adherent primary monocytes expressing EGFP-CAAX, EGFP-OCLN, or EGFP-OCLN-ΔC on hCMEC/D3 monolayer measured by flow cytometry. Adhesion time is 30 min. The data represents the mean values obtained from n = 7 monocyte donors. Data were normalized to the average of EGFP-CAAX obtained from each experiment. Each symbol’s color corresponds to an individual monocyte donor. Error bars are SEM. ( F ) Percentage of transmigrated primary monocytes expressing EGFP-CAAX, EGFP-OCLN, or EGFP-OCLN-ΔC across hCMEC/D3 monolayer. The data were obtained from n = 6 monocyte donors. Data were normalized to the average of EGFP-CAAX obtained from each experiment. Each symbol’s color corresponds to an individual monocyte donor. ( G , H ) 3D time-lapse spinning disk confocal microscopy of transmigrating monocytes. Fluorescence intensity scale is shown on the side of each panel. EGFP is represented using the “red Fire” color-coding, and endothelial cells are stained with a <t>CellTrace</t> marker (cyan) added prior to the addition of the monocytes. Timescales are shown is the bottom right corner and correspond to the time after which imaging acquisition started. ( G ) Imaging of a primary monocyte transduced with EGFP-OCLN on hCMEC/D3 monolayer. Images were taken every 10 min. Scale bar: 10 μm. Full video can be found in Movie . ( H ) Imaging of a THP-1 cell transduced with EGFP-OCLN on hCMEC/D3 monolayer (not stained). Images were taken every 10 min. Scale bar: 8 μm. Full video can be found in Movie . The white square highlights OCLN accumulation at the potential interface between hCMEC/D3 cells and the THP-1 cell. (i) Enlarged image from the white square and a 90° flip showing the accumulation of OCLN. Scale bar: 0.7 µm. Data information: In ( B , C , F ), data are presented as mean ± SEM. In ( D , E ), error bars represent SEM. Two-tailed Student’s t-test p value < 0.05 (*), < 0.01 (**) or non-significant (ns). .
    Celltrace Calcein Red Orange Am C34851, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/celltrace+calcein+red-orange/celltrace+calcein+red+orange+am+c34851/pmc10950437__mmc1-112-9-14
    Average 90 stars, based on 1 article reviews
    celltrace calcein red-orange am c34851 - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    Image Search Results


    ( A ) Heatmap of TJAP-coding mRNAs in human primary monocytes and the THP-1 cell line based on existing RNAseq databases (see Methods). ( B ) siRNA screen on monocytic TJAP proteins to define their role in transmigration. The scheme represents the experimental setup of a transmigration assay with monocytes crossing a confluent monolayer of brain endothelial cells (hCMEC/D3). TJAPs were selected based on the heatmap shown in A. THP-1 cells were electroporated with the indicated siRNAs and added to the top chamber for 17 h (overnight). The data is presented as percentage of transmigrated THP-1 cells normalized to the siRNA control (black line). The graph shows n = 3 independent experiments performed at least in two technical replicates. ( C ) Percentage of transmigrated OCLN KO THP-1 cells rescued either with EGFP-CAAX, EGFP-OCLN, or EGFP-OCLN-ΔC across hCMEC/D3 monolayer. The data were obtained from n = 3 individual experiments performed in two technical replicates normalized to EGFP-CAAX of each experiment. Each symbol’s color corresponds to an individual experiment. Red dashes represent the mean of experiments combined together. ( D ) Percentage of adherent primary monocytes expressing EGFP-CAAX, EGFP-OCLN, or EGFP-OCLN-ΔC on fibronectin substrate measured by flow cytometry. Adhesion time is 30 min. The data were obtained from n = 4 monocyte donors. Data were normalized to the average of EGFP-CAAX obtained from each experiment. Each symbol’s color corresponds to an individual monocyte donor. ( E ) Percentage of adherent primary monocytes expressing EGFP-CAAX, EGFP-OCLN, or EGFP-OCLN-ΔC on hCMEC/D3 monolayer measured by flow cytometry. Adhesion time is 30 min. The data represents the mean values obtained from n = 7 monocyte donors. Data were normalized to the average of EGFP-CAAX obtained from each experiment. Each symbol’s color corresponds to an individual monocyte donor. Error bars are SEM. ( F ) Percentage of transmigrated primary monocytes expressing EGFP-CAAX, EGFP-OCLN, or EGFP-OCLN-ΔC across hCMEC/D3 monolayer. The data were obtained from n = 6 monocyte donors. Data were normalized to the average of EGFP-CAAX obtained from each experiment. Each symbol’s color corresponds to an individual monocyte donor. ( G , H ) 3D time-lapse spinning disk confocal microscopy of transmigrating monocytes. Fluorescence intensity scale is shown on the side of each panel. EGFP is represented using the “red Fire” color-coding, and endothelial cells are stained with a CellTrace marker (cyan) added prior to the addition of the monocytes. Timescales are shown is the bottom right corner and correspond to the time after which imaging acquisition started. ( G ) Imaging of a primary monocyte transduced with EGFP-OCLN on hCMEC/D3 monolayer. Images were taken every 10 min. Scale bar: 10 μm. Full video can be found in Movie . ( H ) Imaging of a THP-1 cell transduced with EGFP-OCLN on hCMEC/D3 monolayer (not stained). Images were taken every 10 min. Scale bar: 8 μm. Full video can be found in Movie . The white square highlights OCLN accumulation at the potential interface between hCMEC/D3 cells and the THP-1 cell. (i) Enlarged image from the white square and a 90° flip showing the accumulation of OCLN. Scale bar: 0.7 µm. Data information: In ( B , C , F ), data are presented as mean ± SEM. In ( D , E ), error bars represent SEM. Two-tailed Student’s t-test p value < 0.05 (*), < 0.01 (**) or non-significant (ns). .

    Journal: EMBO Reports

    Article Title: Targeting monocytic Occludin impairs transendothelial migration and HIV neuroinvasion

    doi: 10.1038/s44319-024-00190-x

    Figure Lengend Snippet: ( A ) Heatmap of TJAP-coding mRNAs in human primary monocytes and the THP-1 cell line based on existing RNAseq databases (see Methods). ( B ) siRNA screen on monocytic TJAP proteins to define their role in transmigration. The scheme represents the experimental setup of a transmigration assay with monocytes crossing a confluent monolayer of brain endothelial cells (hCMEC/D3). TJAPs were selected based on the heatmap shown in A. THP-1 cells were electroporated with the indicated siRNAs and added to the top chamber for 17 h (overnight). The data is presented as percentage of transmigrated THP-1 cells normalized to the siRNA control (black line). The graph shows n = 3 independent experiments performed at least in two technical replicates. ( C ) Percentage of transmigrated OCLN KO THP-1 cells rescued either with EGFP-CAAX, EGFP-OCLN, or EGFP-OCLN-ΔC across hCMEC/D3 monolayer. The data were obtained from n = 3 individual experiments performed in two technical replicates normalized to EGFP-CAAX of each experiment. Each symbol’s color corresponds to an individual experiment. Red dashes represent the mean of experiments combined together. ( D ) Percentage of adherent primary monocytes expressing EGFP-CAAX, EGFP-OCLN, or EGFP-OCLN-ΔC on fibronectin substrate measured by flow cytometry. Adhesion time is 30 min. The data were obtained from n = 4 monocyte donors. Data were normalized to the average of EGFP-CAAX obtained from each experiment. Each symbol’s color corresponds to an individual monocyte donor. ( E ) Percentage of adherent primary monocytes expressing EGFP-CAAX, EGFP-OCLN, or EGFP-OCLN-ΔC on hCMEC/D3 monolayer measured by flow cytometry. Adhesion time is 30 min. The data represents the mean values obtained from n = 7 monocyte donors. Data were normalized to the average of EGFP-CAAX obtained from each experiment. Each symbol’s color corresponds to an individual monocyte donor. Error bars are SEM. ( F ) Percentage of transmigrated primary monocytes expressing EGFP-CAAX, EGFP-OCLN, or EGFP-OCLN-ΔC across hCMEC/D3 monolayer. The data were obtained from n = 6 monocyte donors. Data were normalized to the average of EGFP-CAAX obtained from each experiment. Each symbol’s color corresponds to an individual monocyte donor. ( G , H ) 3D time-lapse spinning disk confocal microscopy of transmigrating monocytes. Fluorescence intensity scale is shown on the side of each panel. EGFP is represented using the “red Fire” color-coding, and endothelial cells are stained with a CellTrace marker (cyan) added prior to the addition of the monocytes. Timescales are shown is the bottom right corner and correspond to the time after which imaging acquisition started. ( G ) Imaging of a primary monocyte transduced with EGFP-OCLN on hCMEC/D3 monolayer. Images were taken every 10 min. Scale bar: 10 μm. Full video can be found in Movie . ( H ) Imaging of a THP-1 cell transduced with EGFP-OCLN on hCMEC/D3 monolayer (not stained). Images were taken every 10 min. Scale bar: 8 μm. Full video can be found in Movie . The white square highlights OCLN accumulation at the potential interface between hCMEC/D3 cells and the THP-1 cell. (i) Enlarged image from the white square and a 90° flip showing the accumulation of OCLN. Scale bar: 0.7 µm. Data information: In ( B , C , F ), data are presented as mean ± SEM. In ( D , E ), error bars represent SEM. Two-tailed Student’s t-test p value < 0.05 (*), < 0.01 (**) or non-significant (ns). .

    Article Snippet: CellTrace Calcein Red-Orange , Invitrogen , C34851.

    Techniques: Transmigration Assay, Control, Expressing, Flow Cytometry, Confocal Microscopy, Fluorescence, Staining, Marker, Imaging, Transduction, Two Tailed Test

    ( A ) Representative scheme of the experimental design associated with the zebrafish model. Human primary monocytes were labeled with CellTrace, and injected into the duct of Cuvier of Tg(fli1a:eGFP-CAAX) zebrafish embryos (GFP-labeled endothelial cells). ( B ) Zebrafish imaging was done at 6–8 h post injection by spinning-disk confocal microscopy. Representative z-projection of confocal images of monocytes from patient X arrested in the vasculature at the tail of a zebrafish embryo at 6 h post injection Scale bar: 100 µm. ( C ) Representative images and orthoslice of monocytes at 6 hpi. Scale bar: 50 µm. ( D ) Violin plots of the ratio of extravasated monocytes from four donors (merged and seperated) at 6–8 h post injection. Each dot represents the ratio calculated from all the monocytes tracked within a fish. The data were obtained from n = 4 individual experiments using four different monocyte donors with nDMSO = 21, nEL1 = 31, nEL2 = 25 in total (nDMSO = 7, nEL1 = 10, nEL2 = 6 for donor W; nDMSO = 6, nEL1 = 7, nEL2 = 4 for donor X; nDMSO = 6, nEL1 = 8, nEL2 = 8 for donor Y; nDMSO = 2, nEL1 = 6, nEL2 = 7 for donor Z). P value < 0.05 (*), and <0.0001 (***). Data information: In ( D ), data are presented as violin plots showing min/max values and quartiles (dotted lines). Statistical difference of non-Gaussian datasets was analyzed using Kruskal–Wallis test with Dunn’s post hoc test p value < 0.05 (*), < 0.001 (***) or non-significant (ns). .

    Journal: EMBO Reports

    Article Title: Targeting monocytic Occludin impairs transendothelial migration and HIV neuroinvasion

    doi: 10.1038/s44319-024-00190-x

    Figure Lengend Snippet: ( A ) Representative scheme of the experimental design associated with the zebrafish model. Human primary monocytes were labeled with CellTrace, and injected into the duct of Cuvier of Tg(fli1a:eGFP-CAAX) zebrafish embryos (GFP-labeled endothelial cells). ( B ) Zebrafish imaging was done at 6–8 h post injection by spinning-disk confocal microscopy. Representative z-projection of confocal images of monocytes from patient X arrested in the vasculature at the tail of a zebrafish embryo at 6 h post injection Scale bar: 100 µm. ( C ) Representative images and orthoslice of monocytes at 6 hpi. Scale bar: 50 µm. ( D ) Violin plots of the ratio of extravasated monocytes from four donors (merged and seperated) at 6–8 h post injection. Each dot represents the ratio calculated from all the monocytes tracked within a fish. The data were obtained from n = 4 individual experiments using four different monocyte donors with nDMSO = 21, nEL1 = 31, nEL2 = 25 in total (nDMSO = 7, nEL1 = 10, nEL2 = 6 for donor W; nDMSO = 6, nEL1 = 7, nEL2 = 4 for donor X; nDMSO = 6, nEL1 = 8, nEL2 = 8 for donor Y; nDMSO = 2, nEL1 = 6, nEL2 = 7 for donor Z). P value < 0.05 (*), and <0.0001 (***). Data information: In ( D ), data are presented as violin plots showing min/max values and quartiles (dotted lines). Statistical difference of non-Gaussian datasets was analyzed using Kruskal–Wallis test with Dunn’s post hoc test p value < 0.05 (*), < 0.001 (***) or non-significant (ns). .

    Article Snippet: CellTrace Calcein Red-Orange , Invitrogen , C34851.

    Techniques: Labeling, Injection, Imaging, Confocal Microscopy

    ( A ) Primary monocytes were infected for 48 h at MOI 1 with HIV-1 (NLAD8) and added to the top chamber of transwells containing a hCMEC/D3 monolayer to allow transmigration to occur. After overnight incubation, the bottom chamber was harvested and the number of transmigrated monocytes was counted. The dot plot shows the mean of the fold change of the number of HIV-exposed monocytes over non-infected counterparts. Each dot corresponds to a donor. ( B ) Primary monocytes were infected for 24 h at MOI 1 with HIV-1 (NLAD8 (R5)). They were added to the top chamber of transwells containing a hCMEC/D3 monolayer to allow transmigration to occur at the same time as cell-free HIV (NLAD8 (R5)) was added to the top chamber of transwells (at the same amount as it was added to monocytes) containing a hCMEC/D3 monolayer for infiltration to happen. After overnight incubation, RNA from the top chamber and the bottom chamber was extracted. The data represents relative HIV RNA level in the bottom chamber (normalized to the top chamber) obtained from individual measurements. The data were obtained from n = 2 donors performed in three technical replicates. ( C ) Schematic of the experimental setup and image representation of time-lapse imaging of primary human monocytes pre-stained with CellTrace (magenta) and exposed to HIV-1 Gag-imCherry (yellow) for 6 h prior to the beginning of the co-incubation of monocytes with a hESC-derived cortical organoid. For representation purposes HIV-1 Gag-imCherry is shown separately in gray scale in the second row. Images were taken every hour for 8 days. At day 0 of co-incubation, monocytes carrying HIV-1 particles show yellow dots (not visible at this resolution). At 18 hpi (corresponding to 0.5 days post-co-incubation, second panel), very few cells were productively infected. Starting from day 4 post co-incubation (panel 3) up until day 7 (panel 4), multiple cells were observed to be productively infected and penetrating inside the cerebral organoid. Scale bar: 50 μm. Full videos can be found in Movie and . This observation is supported by the graph with a pixel sum from a field of view of HIV-1 Gag-imCherry channel showing that pixel sum increases drastically at around 4 days (90–100 h) post co-incubation. ( D ) 3D reconstruction of an immunofluorescent image of a brain organoid with non-infected primary monocytes. Monocyte-derived cells are stained with Iba1 (magenta), astrocytes with GFAP (cyan), and cell nuclei with DAPI (yellow). Scale bar: 30 μm. ( E ) Three-dimensional reconstruction of an immunofluorescence image of a hESC-derived cortical organoid infiltrated with primary monocytes (CellTrace, magenta), infected with HIV-1 Gag-imCherry particles (cyan) for 7 days after the beginning of co-incubation (as in A ). Astrocytes are stained with GFAP (yellow) and cell nuclei with DAPI (blue). Scale bar: 15 μm. The infected monocyte highlighted in the white square is magnified in (i). Scale bar: 5 μm. ( F – H ) Transmigration of primary monocytes exposed to HIV-1 R5 Gag-imCherry for 24 h, washed and pre-incubated with OCLN-derived peptides for 1 h. Monocytes were added at the top of a transwell with a confluent hCMEC/D3 monolayer and transmigration was allowed to happen overnight (≈16 h). ( F ) Schematic of the experimental setup. ( G ) Ratio of the amount of HIV RNA in the supernatant of the lower chamber was divided by the amount measured in the upper chamber. Each dot represents the ratio of the HIV RNA measurement performed in duplicate from a transwell containing a single organoid. The data represents were obtained from n = 4 individual experiments. Each dot represents a biological replicate (varies between conditions). Data normalized to DMSO of each experiment. ( H ) Ratio of the amount of HIV RNA in the organoid of the lower chamber was divided by the amount measured in the upper chamber. Each dot represents the ratio of the HIV RNA measurement performed in duplicate from a transwell containing a single organoid. Each dot represents a biological replicate (varies between conditions). Data normalized to DMSO of each experiment. Data information: In ( A ), the mean is represented with a black line and the normalization to non-infected (fold-change of 1) is marked by a red dotted line. In ( B , G , H ), the data are presented as mean ± SEM. Two-tailed Student’s t-test p value < 0.01 (**) and when not specified between conditions, the difference in not significant. .

    Journal: EMBO Reports

    Article Title: Targeting monocytic Occludin impairs transendothelial migration and HIV neuroinvasion

    doi: 10.1038/s44319-024-00190-x

    Figure Lengend Snippet: ( A ) Primary monocytes were infected for 48 h at MOI 1 with HIV-1 (NLAD8) and added to the top chamber of transwells containing a hCMEC/D3 monolayer to allow transmigration to occur. After overnight incubation, the bottom chamber was harvested and the number of transmigrated monocytes was counted. The dot plot shows the mean of the fold change of the number of HIV-exposed monocytes over non-infected counterparts. Each dot corresponds to a donor. ( B ) Primary monocytes were infected for 24 h at MOI 1 with HIV-1 (NLAD8 (R5)). They were added to the top chamber of transwells containing a hCMEC/D3 monolayer to allow transmigration to occur at the same time as cell-free HIV (NLAD8 (R5)) was added to the top chamber of transwells (at the same amount as it was added to monocytes) containing a hCMEC/D3 monolayer for infiltration to happen. After overnight incubation, RNA from the top chamber and the bottom chamber was extracted. The data represents relative HIV RNA level in the bottom chamber (normalized to the top chamber) obtained from individual measurements. The data were obtained from n = 2 donors performed in three technical replicates. ( C ) Schematic of the experimental setup and image representation of time-lapse imaging of primary human monocytes pre-stained with CellTrace (magenta) and exposed to HIV-1 Gag-imCherry (yellow) for 6 h prior to the beginning of the co-incubation of monocytes with a hESC-derived cortical organoid. For representation purposes HIV-1 Gag-imCherry is shown separately in gray scale in the second row. Images were taken every hour for 8 days. At day 0 of co-incubation, monocytes carrying HIV-1 particles show yellow dots (not visible at this resolution). At 18 hpi (corresponding to 0.5 days post-co-incubation, second panel), very few cells were productively infected. Starting from day 4 post co-incubation (panel 3) up until day 7 (panel 4), multiple cells were observed to be productively infected and penetrating inside the cerebral organoid. Scale bar: 50 μm. Full videos can be found in Movie and . This observation is supported by the graph with a pixel sum from a field of view of HIV-1 Gag-imCherry channel showing that pixel sum increases drastically at around 4 days (90–100 h) post co-incubation. ( D ) 3D reconstruction of an immunofluorescent image of a brain organoid with non-infected primary monocytes. Monocyte-derived cells are stained with Iba1 (magenta), astrocytes with GFAP (cyan), and cell nuclei with DAPI (yellow). Scale bar: 30 μm. ( E ) Three-dimensional reconstruction of an immunofluorescence image of a hESC-derived cortical organoid infiltrated with primary monocytes (CellTrace, magenta), infected with HIV-1 Gag-imCherry particles (cyan) for 7 days after the beginning of co-incubation (as in A ). Astrocytes are stained with GFAP (yellow) and cell nuclei with DAPI (blue). Scale bar: 15 μm. The infected monocyte highlighted in the white square is magnified in (i). Scale bar: 5 μm. ( F – H ) Transmigration of primary monocytes exposed to HIV-1 R5 Gag-imCherry for 24 h, washed and pre-incubated with OCLN-derived peptides for 1 h. Monocytes were added at the top of a transwell with a confluent hCMEC/D3 monolayer and transmigration was allowed to happen overnight (≈16 h). ( F ) Schematic of the experimental setup. ( G ) Ratio of the amount of HIV RNA in the supernatant of the lower chamber was divided by the amount measured in the upper chamber. Each dot represents the ratio of the HIV RNA measurement performed in duplicate from a transwell containing a single organoid. The data represents were obtained from n = 4 individual experiments. Each dot represents a biological replicate (varies between conditions). Data normalized to DMSO of each experiment. ( H ) Ratio of the amount of HIV RNA in the organoid of the lower chamber was divided by the amount measured in the upper chamber. Each dot represents the ratio of the HIV RNA measurement performed in duplicate from a transwell containing a single organoid. Each dot represents a biological replicate (varies between conditions). Data normalized to DMSO of each experiment. Data information: In ( A ), the mean is represented with a black line and the normalization to non-infected (fold-change of 1) is marked by a red dotted line. In ( B , G , H ), the data are presented as mean ± SEM. Two-tailed Student’s t-test p value < 0.01 (**) and when not specified between conditions, the difference in not significant. .

    Article Snippet: CellTrace Calcein Red-Orange , Invitrogen , C34851.

    Techniques: Infection, Transmigration Assay, Incubation, Imaging, Staining, Derivative Assay, Immunofluorescence, Two Tailed Test

    Reagents and tools table

    Journal: EMBO Reports

    Article Title: Targeting monocytic Occludin impairs transendothelial migration and HIV neuroinvasion

    doi: 10.1038/s44319-024-00190-x

    Figure Lengend Snippet: Reagents and tools table

    Article Snippet: CellTrace Calcein Red-Orange , Invitrogen , C34851.

    Techniques: Isolation, Recombinant, Sequencing, Knock-Out, DNA Ligation, Transfection, Cell Viability Assay, Blocking Assay, Protease Inhibitor, Virus, Software, Electroporation, Imaging, Polymer, Microscopy, Flow Cytometry, Membrane